Project

Part:BBa_I737010:Design

Designed by: Dave Curran   Group: iGEM07_Calgary   (2007-10-23)


Light->Sustained GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1058
    Illegal BsaI.rc site found at 2592


Design Notes

The protein LuxR is present in the system twice; it should be expressed first upon exposure to light, and should then activate expression of yet more LuxR. This allows continued expression of GFP even when the light is removed.


Source

This circuit was put together using existing biobrick parts.

References